Blocking Assay:
other:Article Title: Apelin-13 attenuates optic nerve damage in glaucomatous mice by regulating glucose metabolism
Article Snippet: The primary antibodies used were Iba1(1:200, anti-Iba1 antibody, Wako, 019-19741, Japan), NeuN (1:200, anti-NeuN antibody, Cell Signaling Technology, 94403 S, USA), GLUT1 (1:100, Novus, NB110-39113, USA), GLUT3 (1:100, Novus, NBP3-12885, USA), and glucose-6-phosphate dehydrogenase (G6PD) (1:100, GeneTex, GTX101218, USA), followed by secondary antibodies (1:500, Bioworld, China).
Immunohistochemistry:Article Title: Aqp9 Gene Deletion Enhances Retinal Ganglion Cell (RGC) Death and Dysfunction Induced by Optic Nerve Crush: Evidence that Aquaporin 9 Acts as an Astrocyte-to-Neuron Lactate Shuttle in Concert with Monocarboxylate Transporters To Support RGC Function and Survival
Article Snippet: GLUT1 , 1:200, IHC,1:5000, WB , B300-666 , Novus Biologicals , Rabbit. .. GLUT3 , 1:200, IHC, 1:2000, WB , NBP2-66872 , Novus Biologicals , Rabbit. .. MCT1 , 1:500, IHC, 1:1000, WB , bs-10249R , Bioss , Rabbit.
Western Blot:Article Title: Aqp9 Gene Deletion Enhances Retinal Ganglion Cell (RGC) Death and Dysfunction Induced by Optic Nerve Crush: Evidence that Aquaporin 9 Acts as an Astrocyte-to-Neuron Lactate Shuttle in Concert with Monocarboxylate Transporters To Support RGC Function and Survival
Article Snippet: GLUT1 , 1:200, IHC,1:5000, WB , B300-666 , Novus Biologicals , Rabbit. .. GLUT3 , 1:200, IHC, 1:2000, WB , NBP2-66872 , Novus Biologicals , Rabbit. .. MCT1 , 1:500, IHC, 1:1000, WB , bs-10249R , Bioss , Rabbit.
Immunohistochemical staining:Article Title: Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes
Article Snippet: .. 137 b. Localization by Immunohistochemical studies: Placental cryostat sections (10 μm thickness) were 138 subjected to immunofluorescence staining using Glut1 and Glut3 (1:500 dilution each, RRID: 139 AB_2737340 and AB_2631293, a gift from Dr. Takata, Gunma University) and Nestin antibodies (1/50 140 dilution, Neuromics, catalog #GT15114, RRID: AB_1619688) or CaMK2α (1/50, Novus, catalog # NBP1-141 51945, RRID: AB_11037869) and CD34 (endothelial cell marker, 1/100; Abcam, catalog # ab81289, RRID: 142 AB_1640331) as primary antibodies, followed by corresponding secondary antibodies carrying the 143 fluorescent tags of either Alexa 488 (Jackson ImmunoResearch Labs, catalog #705-545-003, RRID: 144 AB_2340428 for Nestin & CaMK2α ; catalog #711-545-152, RRID: AB_2313584 for Glut3 & CD34) or 145 Alexa 594 (Jackson ImmunoResearch Labs, catalog #705-585-003, RRID: AB_2340432 for Nestin in 146 double staining with CD34; catalog #706-585-148, RRID: AB_2340474 for Glut1). ..
Immunofluorescence:Article Title: Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes
Article Snippet: .. 137 b. Localization by Immunohistochemical studies: Placental cryostat sections (10 μm thickness) were 138 subjected to immunofluorescence staining using Glut1 and Glut3 (1:500 dilution each, RRID: 139 AB_2737340 and AB_2631293, a gift from Dr. Takata, Gunma University) and Nestin antibodies (1/50 140 dilution, Neuromics, catalog #GT15114, RRID: AB_1619688) or CaMK2α (1/50, Novus, catalog # NBP1-141 51945, RRID: AB_11037869) and CD34 (endothelial cell marker, 1/100; Abcam, catalog # ab81289, RRID: 142 AB_1640331) as primary antibodies, followed by corresponding secondary antibodies carrying the 143 fluorescent tags of either Alexa 488 (Jackson ImmunoResearch Labs, catalog #705-545-003, RRID: 144 AB_2340428 for Nestin & CaMK2α ; catalog #711-545-152, RRID: AB_2313584 for Glut3 & CD34) or 145 Alexa 594 (Jackson ImmunoResearch Labs, catalog #705-585-003, RRID: AB_2340432 for Nestin in 146 double staining with CD34; catalog #706-585-148, RRID: AB_2340474 for Glut1). ..
Staining:Article Title: Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes
Article Snippet: .. 137 b. Localization by Immunohistochemical studies: Placental cryostat sections (10 μm thickness) were 138 subjected to immunofluorescence staining using Glut1 and Glut3 (1:500 dilution each, RRID: 139 AB_2737340 and AB_2631293, a gift from Dr. Takata, Gunma University) and Nestin antibodies (1/50 140 dilution, Neuromics, catalog #GT15114, RRID: AB_1619688) or CaMK2α (1/50, Novus, catalog # NBP1-141 51945, RRID: AB_11037869) and CD34 (endothelial cell marker, 1/100; Abcam, catalog # ab81289, RRID: 142 AB_1640331) as primary antibodies, followed by corresponding secondary antibodies carrying the 143 fluorescent tags of either Alexa 488 (Jackson ImmunoResearch Labs, catalog #705-545-003, RRID: 144 AB_2340428 for Nestin & CaMK2α ; catalog #711-545-152, RRID: AB_2313584 for Glut3 & CD34) or 145 Alexa 594 (Jackson ImmunoResearch Labs, catalog #705-585-003, RRID: AB_2340432 for Nestin in 146 double staining with CD34; catalog #706-585-148, RRID: AB_2340474 for Glut1). ..
Marker:Article Title: Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes
Article Snippet: .. 137 b. Localization by Immunohistochemical studies: Placental cryostat sections (10 μm thickness) were 138 subjected to immunofluorescence staining using Glut1 and Glut3 (1:500 dilution each, RRID: 139 AB_2737340 and AB_2631293, a gift from Dr. Takata, Gunma University) and Nestin antibodies (1/50 140 dilution, Neuromics, catalog #GT15114, RRID: AB_1619688) or CaMK2α (1/50, Novus, catalog # NBP1-141 51945, RRID: AB_11037869) and CD34 (endothelial cell marker, 1/100; Abcam, catalog # ab81289, RRID: 142 AB_1640331) as primary antibodies, followed by corresponding secondary antibodies carrying the 143 fluorescent tags of either Alexa 488 (Jackson ImmunoResearch Labs, catalog #705-545-003, RRID: 144 AB_2340428 for Nestin & CaMK2α ; catalog #711-545-152, RRID: AB_2313584 for Glut3 & CD34) or 145 Alexa 594 (Jackson ImmunoResearch Labs, catalog #705-585-003, RRID: AB_2340432 for Nestin in 146 double staining with CD34; catalog #706-585-148, RRID: AB_2340474 for Glut1). ..
Double Staining:Article Title: Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes
Article Snippet: .. 137 b. Localization by Immunohistochemical studies: Placental cryostat sections (10 μm thickness) were 138 subjected to immunofluorescence staining using Glut1 and Glut3 (1:500 dilution each, RRID: 139 AB_2737340 and AB_2631293, a gift from Dr. Takata, Gunma University) and Nestin antibodies (1/50 140 dilution, Neuromics, catalog #GT15114, RRID: AB_1619688) or CaMK2α (1/50, Novus, catalog # NBP1-141 51945, RRID: AB_11037869) and CD34 (endothelial cell marker, 1/100; Abcam, catalog # ab81289, RRID: 142 AB_1640331) as primary antibodies, followed by corresponding secondary antibodies carrying the 143 fluorescent tags of either Alexa 488 (Jackson ImmunoResearch Labs, catalog #705-545-003, RRID: 144 AB_2340428 for Nestin & CaMK2α ; catalog #711-545-152, RRID: AB_2313584 for Glut3 & CD34) or 145 Alexa 594 (Jackson ImmunoResearch Labs, catalog #705-585-003, RRID: AB_2340432 for Nestin in 146 double staining with CD34; catalog #706-585-148, RRID: AB_2340474 for Glut1). ..
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